FSCB phosphorylation in mouse spermatozoa capacitation

Tyrosine Phosphorylation Events in Mouse Sperm Capacitation

Endogenous redox activity in mouse spermatozoa and its role in regulating the tyrosine phosphorylation events associated with sperm capacitation.

We investigated the role of endogenous redox activity in regulating the signal transduction pathway leading to tyrosine phosphorylation in mouse spermatozoa. Endogenous redox activity was monitored using a luminol-peroxidase chemiluminescent probe. Chemiluminescence increased in spermatozoa that were actively undergoing cAMP-mediated tyrosine phosphorylation events associated with capacitation ...

Effects of Ca and HCO3 on Capacitation, Hyperactivation and Protein Tyrosine Phosphorylation in Guinea Pig Spermatozoa

In our previous report, we demonstrated that the tyrosine phosphorylation of sperm proteins (TPSP) of guinea pig was associated with capacitation and hyperactivation (CAHA), and Ca and HCO3 were required for the initiation of CAHA and increasing the TPSP. The aim of this study was to further investigate the mechanism underlying the above events. The results showed that addition of cAMP agonists...

Identification of proteins undergoing tyrosine phosphorylation during mouse sperm capacitation.

Mammalian sperm are not able to fertilize immediately upon ejaculation; they become fertilization-competent after undergoing changes in the female reproductive tract collectively termed capacitation. Although it has been established that capacitation is associated with an increase in tyrosine phosphorylation, little is known about the role of this event in sperm function. In this work we used a...

Cat (Felis catus L.) spermatozoa require capacitation.

In the oestrous female cat, fertilization can take place when spermatozoa are introduced into the vagina up to 49 hr after an ovulation-inducing injection of human chorionic gonadotrophin. The spermatozoa require residence in the female reproductive tract before they are capable of fertilizing the eggs in vitro. No eggs were fertilized by freshly ejaculated spermatozoa. One of four oestrous fem...